Clinical Significance of the Detection of CD55, CD59 Expression Deletion in Patients with Cytopenia_West China Medical Journal

Authors：

LUuml ¹ ,  Min ² , ZHU Huanling ³ , JIANG Nenggang ³

1. West China Medical School; 2. Department of Hematology, West China Hospital; 3. Laboratory Medicine Department; West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

Min, Email: lv1985518@126.com

Keywords：

血细胞减少; 阵发性睡眠性血红蛋白尿; 衰变加速因子; 膜反应性溶血抑制物

DOI：

CNKI: 51-1356/R.20120214.1338.001

Video：

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目的　检测血细胞减少患者外周血红细胞和中性粒细胞细胞膜糖基磷脂酰肌醇（GPI）连接的补体调节蛋白衰变加速因子（CD55)和膜反应性溶血抑制物（CD59）表达情况，并探讨其临床意义。方法　2006年7月－2011年3月，采用直接免疫荧光标记法流式细胞仪检测182例血细胞减少患者外周血CD55及CD59表达情况，其中阵发性睡眠性血红蛋白尿（PNH）9例，再生障碍性贫血（AA）-PNH综合征8例，AA 83例，骨髓增生异常综合征51例，自身免疫性溶血性贫血11例，造血功能停滞6例，缺铁性贫血7例，巨幼细胞性贫血4例，脾功能亢进3例。结果　 PNH及AA-PNH患者CD55、CD59抗原缺失率均较其他血细胞减少者明显增高。结论　流式细胞仪检测外周血中红细胞和中性粒细胞膜CD55和CD59抗原表达缺失率是目前诊断PNH可靠和敏感的方法，也是对PNH、AA-PNH早期诊断敏感指标，并且PNH克隆检测还能为诊断疾病提供鉴别诊断依据。

Citation： LUuml,Min,ZHU Huanling,JIANG Nenggang,ZENG Tingting. Clinical Significance of the Detection of CD55, CD59 Expression Deletion in Patients with Cytopenia. West China Medical Journal, 2012, 27(2): 163-167. doi: CNKI: 51-1356/R.20120214.1338.001 Copy

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2.	Piedras J, Lopez KX. Flow cytometric analysis of glyeosylphosphatidylinositol-anchored proteins to assess paroxysmal nocturnal hemoglobinuria clone size[J]. Cytometry, 2000, 42(4): 234-238.
3.	Oelschlaegel U, Besson I, Arnoulet C, et al. A standardized flow cytometric method for screening paroxysmal nocturnal hemoglobinuria (PNH) measuring CD55 and CD59 expression on erythrocytes and granulocytes[J]. Clin Lab Haematol, 2001, 23(2): 81-90.
4.	高珏华. CD59和阵发性睡眠性血红蛋白尿症[J]. 国外医学·输血及血液学分册, 1998, 21(6): 380-384.
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6.	Okuda K, Kanamaru, AUeda E, et al. Membrane expression of decay-accelerating factor on neutrophil from normal individuals and patients with paroxysmal nocturnal hemoglobinuria[J]. Blood, 1990, 75(5): 1186-1191.
7.	朱明清, 耿美菊, 陈黎, 等. CD55、CD59检测阵发性睡眠性血红蛋白尿症标准方法建立[J]. 中国血液流变学杂志, 2006, 16(3): 447-448.
8.	Hall E, Rosse W. The use of monoclonal antibodies and flow cytometry in diagnosis of PNH [J].Blood, 1996, 87(2): 5332-5340.
9.	金冬岩, 陆春伟, 佟海侠, 等. 流式细胞术检测贫血患者血细胞CD55和CD59的表达及临床意义[J]. 中国医学工程, 2011, 19(6): 161-162.
10.	丁磊, 胡翊群, 姜叙诚. 健康人群外周血各类血细胞CD55/CD59的表达[J]. 检验医学, 2009, 24(3): 205-207.
11.	徐裕海, 礼征楠, 王永才, 等. CD55、CD59免疫表型对PNH相关疾病诊断的观察研究[J]. 中国医疗前沿, 2010, 5(3): 71-72.
12.	陈婷. AA发展为PNH、MDS、AML 1例[J]. 现代诊断与治疗, 1997, 8(1): 25-26.
13.	Sugimori C, Chuhjo T, Feng XM, et al. Minor population of CD55- CD59- blood cells predicts response to immunosuppessive therapy and prognosis in patients with aplastic anemia[J]. Blood, 2006, 107(4): 1308-1314.
14.	Young NS. The problem of clonality in aplastic anemia: Dr. Dameshek’s riddle, restated[J]. Blood, 1992, 79(9): 1385-1392.
15.	王婷, 邵宗鸿. 再生障碍性贫血相关的恶性疾病及克隆改变[J]. 实用医院临床杂志, 2011, 8(4): 1-4.
16.	Maciejewski JP, Rivera C, Kook H, et al. Relationship between bone marrow failure syndromes and the presence of glycosylphosphatidylinositol anchored protein-deficient clones[J]. Br J Haematol, 2001, 115(4): 1015-1022.
17.	Dunn DE, Tanawattanacharoen P, Boccuni P, et al. Paroxysmal nocturnal hemoglobinuria cell in patients with bone marrow failure syndromes[J]. Ann Intern Med, 1999, 131(6): 401-408.
18.	李玉云, 王瑞英, 杨吉成. 骨髓增生异常综合征患者外周血细胞免疫表型分析[J]. 蚌埠医学院学报, 2003, 28(3): 198-200.
19.	邵宗鸿. 自身免疫性溶血性贫血与阵发性睡眠性血红蛋白尿症患者的输血治疗[J]. 中国实用内科杂志, 2004, 24(9): 515-516.
20.	方永光, 向阳, 成玉斌, 等. 自身免疫性溶血性贫血伴阵发性睡眠性血红蛋白尿1例[J]. 临床血液学杂志, 2009, 22(1): 51.

1. 张之南, 沈悌. 血液病诊断及疗效标准[M]. 3版. 北京: 科学出版社, 2011: 19-54.
2. Piedras J, Lopez KX. Flow cytometric analysis of glyeosylphosphatidylinositol-anchored proteins to assess paroxysmal nocturnal hemoglobinuria clone size[J]. Cytometry, 2000, 42(4): 234-238.
3. Oelschlaegel U, Besson I, Arnoulet C, et al. A standardized flow cytometric method for screening paroxysmal nocturnal hemoglobinuria (PNH) measuring CD55 and CD59 expression on erythrocytes and granulocytes[J]. Clin Lab Haematol, 2001, 23(2): 81-90.
4. 高珏华. CD59和阵发性睡眠性血红蛋白尿症[J]. 国外医学·输血及血液学分册, 1998, 21(6): 380-384.
5. Pilar MH, Marta MA, Julia A, et al. Comparative analysis of different flow cytometry based immuophenotypic methods for the analysis of CD55 and CD59 expression on major peripheral blood cell subsets[J]. Cytometry, 2002, 50(3): 191-201 .
6. Okuda K, Kanamaru, AUeda E, et al. Membrane expression of decay-accelerating factor on neutrophil from normal individuals and patients with paroxysmal nocturnal hemoglobinuria[J]. Blood, 1990, 75(5): 1186-1191.
7. 朱明清, 耿美菊, 陈黎, 等. CD55、CD59检测阵发性睡眠性血红蛋白尿症标准方法建立[J]. 中国血液流变学杂志, 2006, 16(3): 447-448.
8. Hall E, Rosse W. The use of monoclonal antibodies and flow cytometry in diagnosis of PNH [J].Blood, 1996, 87(2): 5332-5340.
9. 金冬岩, 陆春伟, 佟海侠, 等. 流式细胞术检测贫血患者血细胞CD55和CD59的表达及临床意义[J]. 中国医学工程, 2011, 19(6): 161-162.
10. 丁磊, 胡翊群, 姜叙诚. 健康人群外周血各类血细胞CD55/CD59的表达[J]. 检验医学, 2009, 24(3): 205-207.
11. 徐裕海, 礼征楠, 王永才, 等. CD55、CD59免疫表型对PNH相关疾病诊断的观察研究[J]. 中国医疗前沿, 2010, 5(3): 71-72.
12. 陈婷. AA发展为PNH、MDS、AML 1例[J]. 现代诊断与治疗, 1997, 8(1): 25-26.
13. Sugimori C, Chuhjo T, Feng XM, et al. Minor population of CD55- CD59- blood cells predicts response to immunosuppessive therapy and prognosis in patients with aplastic anemia[J]. Blood, 2006, 107(4): 1308-1314.
14. Young NS. The problem of clonality in aplastic anemia: Dr. Dameshek’s riddle, restated[J]. Blood, 1992, 79(9): 1385-1392.
15. 王婷, 邵宗鸿. 再生障碍性贫血相关的恶性疾病及克隆改变[J]. 实用医院临床杂志, 2011, 8(4): 1-4.
16. Maciejewski JP, Rivera C, Kook H, et al. Relationship between bone marrow failure syndromes and the presence of glycosylphosphatidylinositol anchored protein-deficient clones[J]. Br J Haematol, 2001, 115(4): 1015-1022.
17. Dunn DE, Tanawattanacharoen P, Boccuni P, et al. Paroxysmal nocturnal hemoglobinuria cell in patients with bone marrow failure syndromes[J]. Ann Intern Med, 1999, 131(6): 401-408.
18. 李玉云, 王瑞英, 杨吉成. 骨髓增生异常综合征患者外周血细胞免疫表型分析[J]. 蚌埠医学院学报, 2003, 28(3): 198-200.
19. 邵宗鸿. 自身免疫性溶血性贫血与阵发性睡眠性血红蛋白尿症患者的输血治疗[J]. 中国实用内科杂志, 2004, 24(9): 515-516.
20. 方永光, 向阳, 成玉斌, 等. 自身免疫性溶血性贫血伴阵发性睡眠性血红蛋白尿1例[J]. 临床血液学杂志, 2009, 22(1): 51.

West China Medical Journal

Clinical Significance of the Detection of CD55, CD59 Expression Deletion in Patients with Cytopenia

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